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  Home > About VDL > News > Test Updates and Announcements
 

Test Updates and Announcements

 

**VDL Endocrinology Laboratory Announcement: Important Changes**
(If you have questions related to these changes, please call the Endocrinology Lab at 612-624-0761.)

As of June 25, 2010 the Endocrinology Laboratory will no longer offer clinical endocrinology service based on single sample submissions and daily scheduling. In light of the current economic environment, and realizing that routine diagnostic testing is available at many commercial laboratories, we will now focus on the needs of research investigators who require test validation or the determination of endocrine/metabolic analytes in samples that can be batched for cost effective service. 

Samples submitted after June 25th will be forwarded to approved VDL contract laboratories for analysis, interpretation, reporting and archiving of results.

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VDL starts using Oral Fluid Idexx ELISA kit for PRRS virus

Earlier this year, Idexx, Europe launched ELISA kit for antibody detection against porcine reproductive and respiratory syndrome (PRRS) virus in oral fluid of pigs.  Recently, VDL has started to use this kit.  Previously according to ISU protocol, antibody kit for PRRS detection in serum of pigs was being validated for oral fluids.

In the year 2012, VDL participated in field trial conducted by Idexx for USDA approval of this kit.  The validation results are available by clicking here.  There was overall 100% agreement between Idexx and MN-VDL results for 20- sample set across three different kit lots.

 

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Serology -- VDL Now Offering Oral Fluid ELISA for PRRS Antibodies (1/18/12)

VDL is now offering an ELISA on oral fluid samples for detecting antibodies against porcine reproductive and respiratory syndrome virus (PRRSV). The test uses Herdcheck X3 ELISA kit (Idexx) for detecting antibodies in serum samples. The test has been validated by Iowa State University (ISU) researchers and has been reported to have an estimated sensitivity and specificity of 94.7% and 100% respectively at Herdchek X3 cut off of ≥ 0.40.

Recently, our laboratory participated in an interlaboratory reproducibility study using a panel of 264 oral fluid samples. A total of 10 laboratories participated in the study initiated by ISU. Our laboratory results were found to be 100% in agreement with mean of results from the other 9 participating laboratories.

Disclaimer: In preliminary trials, it has been found that oral fluid collected from pigs that are on transition diets including spray dried plasma containing anti-PRRSV antibody can show false positive reaction in PRRS ELISA (Zimmerman, Personal communications, 2011).

The test will be offered starting from Wednesday, January 18, 2012. It is a two days test with overnight incubation. The test will be set up on M-Th and results will be reported T-F. More information.

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Immunohistochemistry -- USDA No Longer Paying for Chronic Wasting Disease Testing (1/6/12)


More information

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Serology -- VDL Resumes Offering Idexx APP IV ELISA Testing (11/21/11)

The VDL will start offering Idexx APP-ApxIV ELISA for detecting antibodies against Actinobacillus pleuropneumoniae (APP) from Monday, 11/21/11. The test kit has become available again through Idexx. The test will be performed on Mondays and Thursdays every week, and the fee is $11/per sample.

We evaluated the test kit by testing and comparing results of samples that were tested in Montreal lab for multi APP antibodies. The new test compares well with this test and is 100% specific. With VDL offering this test, samples will no longer have to be sent to the Montreal lab, resulting in quicker turnaround times. For more information, see Services and Fees.

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Serology -- VDL Begins Testing for Canine Influenza (11/01/11)

Canine flu, caused by type A influenza virus, is a newly emerging pathogen in dogs. The canine influenza virus is an H3N8 serotype of the influenza virus. The H3N8 influenza virus has existed in horses for years. The virus has now adapted itself to dogs and can be spread between dogs.

UMN VDL will start offering hemagglutination inhibition (HI) test for detecting antibodies to H3N8 canine influenza virus in serum samples of dogs. The test has been validated in our laboratory using a panel of serum samples obtained from another laboratory, which has considerable experience in H3N8 testing. We will start offering the test starting November 1, 2011. The cost of testing is $10/sample and the test will be performed on a weekly basis on Wednesdays.

More information

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Aquaculture/Fisheries -- Viral Hemorrhagic Septicemia (VHS) Surveillance Study Updated (9/14/11)

More information
2011 VHS Surveillance Sites and Results

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Serology -- PRV Latex Agglutination Test to be Discontinued (8/22/11)

Starting Monday, September 19, VDL will no longer offer latex agglutination test (Autolex) for pseudorabies (PRV) antibodies in swine serum samples.

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Molecular Diagnostics -- Myocbacterium paratuberculosis (Johne's) AGID Test to be Discontinued (7/5/11)

VDL will no longer offer Johne's AGID testing, effective 7/28/11, since Immucell Corp. has discontinued manufacturing the Rapid Johne's Kit used for this test.

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Bacteriology -- New Fees for Susceptibility Testing (12/29/10)

Effective January 1, 2011, the Veterinary Diagnostic Laboratory will begin to charge a nominal fee of $18.00 per isolate for susceptibility testing.  Implementation of the fee will help the VDL better manage the cost of testing multiple bacterial pathogens identified in disease diagnostics.  Previously, susceptibility testing was included in the base price for ‘General Examination of Tissue’ and ‘Necropsy’.  Susceptibility testing of bacteria isolated from various tissues is performed to determine if a bacterium is likely to be susceptible to the action of various antimicrobial agents at dose levels that can be achieved therapeutically.  Sensitivity testing is best used for organisms that are known to be pathogens and that show considerable variation in sensitivity to individual antimicrobial agents.  The University of Minnesota Veterinary Diagnostic Laboratory utilizes the Trek Sensititre Sensitivity system for testing of animal pathogens, which enables semi-automated, rapid, accurate testing of bacterial isolates and direct determination of minimum inhibitory concentrations (MIC) of each isolate.  This system includes up to 40 antimicrobial compounds that are commonly used in veterinary medicine and is the system used for global surveillance programs.

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Serology -- New ELISA test for Haemophilus parasuis (12/14/10)

The VDL is offering a new test (ELISA) for detecting antibodies against Haemophilus parasuis (OppA) in serum of pigs using OppA Antibody Test Kit from Biochek, Netherlands. The test is done once a week (Wednesdays). Click here for fee and submission information.

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Molecular Diagnostics -- New PCR Testing for Mycoplasma Synoviae (12/7/10)

Submission Information
Mycoplasma Synoviae Fact Sheet

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Serology -- Brucella Ring Test (BRT) Discontinued (10/19/10)

Starting November 1, 2010, VDL will no longer offer Brucella Ring Test (BRT) used to test for antibodies in milk samples of ruminants. All submissions will be outsourced to National Veterinary Services Lab (NVSL) after this date. Please refer to updated information under Bovine Serology and Caprine Serology in our Services and Fees section.

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Endocrinology -- Serum Growth Hormone Test Discontinued (8/24/10)

As of August 24, 2010, the VDL  Endocrinology/Metabolic/Cytokine Lab will no longer be able to offer the serum growth hormone test validated for use in the dog and the cat. Unfortunately, the test kit manufacturer discontinued production without any advance notice to its clients. Therefore, we are unable to provide service and have not been able to identify a method for substitution.  We suggest that clients consider use of the insulin like growth factor-1 (IGF-1) test to indirectly evaluate GH production in these animals.  This test is offered at Michigan State Diagnostic Center for Population and Animal Health (DCPAH, 517-353-1683).

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Serology -- Mycoplasma Test Discontinued (8/4/10)

Starting August 17, 2010, VDL will no longer offer Mycoplasma hyopneumoniae Tween-20 ELISA.

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Serology -- New Brucella FPA Test (8/2/10)

Starting August 2, 2010, VDL will widen its scope for Brucella serology by offering Fluorescence Polarization Assay (FPA) on swine samples. Recently, the laboratory participated in 2010 Brucella proficiency testing by National Veterinary Services Laboratory for this test and got approved with 100% success rate. Click here for fee and submission information.

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CVM VBS Mickelson Lab_Leo with Stick 
Molecular Diagnostics -- New Test for Leonberger Polyneuropathy (7/1/10)

The VDL is now offering genetic testing for Leonberger Polyneuropathy (LPN1). Please see the LPN1 home page for information on the condition and how to submit samples for testing.

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Endocrinology -- Routine Testing Discontinued (5/27/10)

As of June 25, 2010 the Endocrinology Laboratory will no longer offer endocrinology service for routine diagnostic testing. In light of the current economic environment, and realizing that routine diagnostic testing is available at many commercial laboratories, we are choosing to redefine our mission to focus on the needs of research investigators who require non-traditional testing and/or test validation.

Samples received after June 25th will be forwarded to approved VDL contract laboratories for analysis.

Determination of serum growth hormone concentration will continue to be available on a monthly basis. If you have questions related to these changes, please call the Endocrinology Lab at 612-624-0761.

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Serology -- New ELISA Test Kit for PRRS Antibodies (5/24/10)

Effective June 1, 2010, University of Minnesota Veterinary Diagnostic Laboratory (VDL) will start offering new HerdChek X3 ELISA for detection of antibodies to Porcine Reproductive and Respiratory Syndrome (PRRS) Virus.  The test will replace the PRRX 2XR test being offered currently. The new test has been reported by Idexx to have 99.9 % specificity and 98.8 % sensitivity. Other advantages cited for the new kit are: a 90% reduction in false positive (Singleton) reactors and early detection (8-14 days post infection) of PRRS antibodies (HerdChek PRRS X3 information sheet:  Idexx).

More Information

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Serology -- New PRV Surveillance Program (5/24/10)

More Information

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Serology -- New Test for Johne's Disease (5/10/10)

Production Animal Services at Idexx has released a new Mycobacterium paratuberculosis (Johne’s disease) Antibody Test Kit for the detection of antibodies in serum, milk and plasma samples. University of Minnesota Veterinary Diagnostic Laboratory will start using this kit effective July 1, 2010.

More Information

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Molecular Diagnostics -- New Test for Canine Exercise Induced Collapse (7/28/08)

Click here for more information on how to submit samples for this test.

 

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