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Viral PCR
| Effective January 1, 2009 | | |
Note: A $10 accession fee and a $10 hazardous waste disposal fee will be applied to all submissions. All fees are in US $. |
| Click the name of the test or procedure for detailed information. |
| Avian Pneumovirus - APV | | Fee: 6.00 | Specimen: tracheal swabs | Shipping Container: 15 ml screw cap vial (5 swabs/vial) | Coolant: frozen gel packs | Days Tested:
once monthly | Turnaround Time:
up to 4 weeks | | Comments: 10 swabs used per test. Call lab for testing schedule. |
Top of Page | Bovine Viral Diarrhea - BVD | Fee:
1) 6.00
2) 16.00 | Specimen: 1) serum (at least 0.5 ml)
2) tissue | Shipping Container: 1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag | Coolant: frozen gel packs | Days Tested:
M,W | Turnaround Time:
next day (from test date) | | Comments: |
Top of Page | Bovine Viral Diarrhea - BVD Ear Notch Screen Pooled Testing | | Fee: 29.00 | Specimen: 1cm x 1cm ear tissue
(1/2 inch notching pliers) | Shipping Container: 6 or 10 ml red top tube (no additive or gel) | Coolant: frozen gel packs | Days Tested:
M,W | Turnaround Time:
1-2 days | | Comments: Samples will be run in pools up to 50. See Virology for BVD ear notch individual testing. Additional information. |
Top of Page | Bovine Viral Diarrhea - BVD Genetic Analysis | | Fee: 138.75 | Specimen: 1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy) | Shipping Container: 1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
weekly | Turnaround Time:
2-4 weeks | Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery. |
Top of Page | Bovine Viral Diarrhea - BVD, Individual or Pooled Samples (for Alpacas and Llamas only). | Fee:
27.50 (individual)
6.00 (pool) | Specimen: 1) serum
2) tissue
3) buffy coat | Shipping Container: 1) leakproof 1.5 ml microfuge tube
2) whirl pac or plastic bag
3) 3-5 ml purple top whole blood tube | Coolant: frozen gel packs | Days Tested:
M,W | Turnaround Time:
next day | | Comments: Results will be reported as individual animals. |
Top of Page | Circovirus Universal (Types 1 and 2) | | Fee: 41.25 | Specimen: 1) serum (at least 0.5 ml)
2) tissue
3) feces | Shipping Container: 1) leakproof 1.5 ml microfuge tube
2,3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
T,F | Turnaround Time:
1-2 days (from test date) | | Comments: |
Top of Page | Circovirus Type 2 | | Fee: 34.50 | Specimen: 1) semen (at least 0.5 ml)
2) serum (at least 0.5 ml)
3) tissue
4) blood swabs
5) feces
6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,4) leakproof 1.5 ml microfuge tube
2) red top tube
3,5) whirl-pac or plastic bag
6) sterile tube or whirl pac
| Coolant: frozen gel packs | Days Tested:
T,F | Turnaround Time:
1-2 days (from test date) | | Comments: 6) Do not pool group/pen samples. |
Top of Page | Circovirus Type 2 Genetic Analysis | | Fee: 126.00 | Specimen: 1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag
4) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested:
weekly | Turnaround Time:
2-4 weeks | Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples. |
Top of Page | Encephalomyocarditis Virus - EMC | | Fee: 30.00 | Specimen: 1) tissue
2) virus isolate | Shipping Container: 1) whirl-pac or plastic bag
2) leakproof 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested:
| Turnaround Time:
5 days | | Comments: |
Top of Page | Equine Encephalidites | | Fee: no charge | Specimen: brain tissue | Shipping Container: | Coolant: | Days Tested:
daily | Turnaround Time:
4 days | | Comments: Sent to Minnesota Dept. of Health. |
Top of Page | Exotic Newcastle Disease Matrix (USDA) | | Fee: 40.50 | Specimen: tracheal swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested:
W | Turnaround Time:
1-2 days (from test date) | | Comments: Swabs may be pooled up to 5. |
Top of Page | Exotic Newcastle Disease Fusion (USDA) | | Fee: 40.50 | Specimen: tracheal swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested:
W | Turnaround Time:
1-2 days (from test date) | | Comments: Swabs may be pooled up to 5. Fusion PCR is run to detect virulent NDV after a positive is obtained by END virus Matrix PCR testing. |
Top of Page | Hemagglutinating Encephalomyelitis Virus - HEV (VWD) | | Fee: 30.00 | Specimen: 1) tissue
2) virus isolate
| Shipping Container: 1) whirl-pac or plastic bag
2) leakproof 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested:
| Turnaround Time:
5 days | | Comments: |
Top of Page | Infectious Bovine Rhinotracheitis - IBR/BHV 1 | | Fee: 22.00 | Specimen: 1) swabs (nasal, conjunctival, vaginal or preputial)
2) lung or other tissue
3) virus isolate
4) tracheal wash or lavage | Shipping Container: 1) synthetic swabs
2) whirl-pac or plastic bag
3) leakproof 1.5 ml microfuge tube
4) sterile tube
| Coolant: 1,2,4) frozen gel packs
3) dry ice | Days Tested:
T | Turnaround Time:
same day (as test date) | | Comments: Results reported on Tuesdays and Thursdays (samples must be recieved at least the day before test date). |
Top of Page | Influenza Virus - Group A Matrix RRT - PCR | | Fee: 26.75 | Specimen: 1) nasal swabs
2) lung (2-3 cm3 or biopsy)
3) oral fluids/saliva (at least 2 ml) | Shipping Container: 1) synthetic swab
2,3) whirl-pac or plastic bag
| Coolant: frozen gel packs | Days Tested:
M,T,W,F | Turnaround Time:
2-3 days | Comments:
2) Shipping instruction: send next day delivery.
3) Do not pool group/pen samples. |
Top of Page | Influenza Virus - Group A Matrix (USDA) | | Fee: 26.75 | Specimen: swabs | Shipping Container: synthetic swab | Coolant: frozen gel packs | Days Tested:
M,T,W,F | Turnaround Time:
2 days | | Comments: For Avians. Swabs may be pooled up to 5. Results reported same day as test date. |
Top of Page | Influenza Virus - Group A, Subtyping for H & N | | Fee: 20.25 | Specimen: 1) nasal swabs
2) virus isolate (at least 0.5 ml)
3) lung (2-3 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml)
| Shipping Container: 1) synthetic swab
2) leakproof 1.5 ml microfuge tube
3) whirl-pac or plastic bag | Coolant: 1,3) frozen gel packs
2) dry ice | Days Tested:
W | Turnaround Time:
1-2 weeks | Comments:
Subtyping tests are run on samples previously testing positive for influenza virus.
2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples. |
Top of Page | Influenza Virus - Group A, Subtyping for H5/H7 (USDA) | | Fee: 14.00 | Specimen: swabs | Shipping Container: synthetic swabs | Coolant: frozen gel packs | Days Tested:
M,W | Turnaround Time:
24 hrs. (from test date) | | Comments: Testing for subtyping is run after a positive is obtained by Influenza virus Matrix testing. |
Top of Page | Influenza Virus - USDA Differential N1 | | Fee: no charge | Specimen: 1) matrix influenza PCR positive sample
2) virus isolate
| Shipping Container: 1) sterile tube
2) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested:
| Turnaround Time:
| | Comments: Requires a written agreement with the USDA. |
Top of Page | Influenza Virus Sequencing | | Fee: 138.75 | Specimen: 1) nasal swabs
2) viral isolate (at least 0.5 ml)
3) lung (2-3 cm3 or biopsy)
4) oral fluids/saliva (at least 2 ml) | Shipping Container: 1) synthetic swab
2) leakproof 1.5 ml microfuge tube
3,4) whirl-pac or plastic bag | Coolant: 1,3,4) frozen gel packs
2) dry ice | Days Tested:
weekly | Turnaround Time:
2-4 weeks | Comments:
2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples. |
Top of Page | Largemouth Bass Virus | | Fee: 25.00 | Specimen: 1) tissue
2) culture media | Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested:
M-F | Turnaround Time:
3-5 days | | Comments: |
Top of Page | Porcine Cytomegalovirus - PCMV | | Fee: 57.75 | Specimen: 1) nasal turbinates
2) nasal swabs
3) buffy coat | Shipping Container: | Coolant: frozen gel packs | Days Tested:
M | Turnaround Time:
2-7 days (from test date) | | Comments: Samples should be received by the previous Friday. |
Top of Page | Porcine Parvovirus - PPV | | Fee: ref. lab fee | Specimen: 1) fetal thoracic fluid
2) tissue | Shipping Container: 1) leakproof container
2) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
| Turnaround Time:
| | Comments: Sent to reference lab. |
Top of Page | Porcine Reproductive and Respiratory Virus - NAEU PRRS | | Fee: 25.75 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)
4) blood swabs
5) nasal swabs
6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag
5) synthetic swab
6) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested:
M-F | Turnaround Time:
same day (boar stud monitoring)
or next day (regular request) Holiday schedule for boar stud monitoring | Comments:
3) Shipping instruction: send next day delivery.
6) Do not pool group/pen samples.
The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information. |
Top of Page | Porcine Reproductive and Respiratory Virus - NAEU Differential PRRS | | Fee: 25.75 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)
4) blood swabs
5) nasal swabs
6) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag
5) synthetic swab
6) sterile tube or whirl pac
| Coolant: frozen gel packs | Days Tested:
weekly | Turnaround Time:
1-7 days | Comments:
3) Shipping instruction: send next day delivery.
6) Do not pool group/pen samples.
The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information. |
Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Quantitative (U.S. Strain Only) | | Fee: 30.00 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy) | Shipping Container: 1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
T | Turnaround Time:
1-2 days (from test date) | Comments:
3) Shipping instruction: send next day delivery.
Additional information. |
Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Genetic Analysis (Strain Differentiating/Sequencing) | | Fee: 126.00 | Specimen: 1) serum (at least 300 uL)
2) virus isolate (at least 300 uL)
3) tissue (1 cm3 or biopsy)
4) extracted RNA
5) oral fluids/saliva (at least 2 ml) | Shipping Container: 1,2) 1.5 ml microfuge tube
3) whirl-pac or plastic bag
4) sterile tube
5) sterile tube or whirl pac | Coolant: frozen gel packs | Days Tested:
weekly | Turnaround Time:
2-4 weeks | Comments:
2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
5) Do not pool group/pen samples.
Additional info. |
Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Tetracore European | | Fee: 27.25 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)
4) blood swabs | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
W | Turnaround Time:
1 day (from test day) | | Comments: 3) Shipping instruction: send next day delivery. Additional Information . |
Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Tetracore Muliplex | | Fee: 45.25 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue
4) blood swabs | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl-pac or plastic bag | Coolant: frozen gel packs | Days Tested:
W | Turnaround Time:
1 day (from test day) | | Comments: 3) Shipping instruction: send next day delivery. Additional Information. |
Top of Page | Porcine Reproductive and Respiratory Virus - PRRS, Tetracore US | | Fee: 27.25 | Specimen: 1) semen (at least 1 ml)
2) serum (at least 0.5 ml)
3) tissue (1 cm3 or biopsy)
4) blood swabs | Shipping Container: 1,2,4) leakproof, 1.5 ml microfuge tube
3) whirl pac or plastic bag | Coolant: frozen gel packs | Days Tested:
W | Turnaround Time:
1 day (from test day) | | Comments: 3) Shipping instruction: send next day delivery. Additional Information . |
Top of Page | Porcine Rotavirus (Types A and C) | | Fee: 25.00 | Specimen: 1) feces
2) intestine
3) feedback material
4) environmental swabs
5) virus isolate | Shipping Container: 1,2,3) whirl-pac or leakpoof container
4) swab
5) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested:
M,W,F | Turnaround Time:
3 days | | Comments: |
Top of Page | Porcine Rotavirus Sequencing - Type C (VP7) and Type A (VP7 and VP4) | | Fee: 126.00 | Specimen: 1) PCR positive sample
2) virus isolate | Shipping Container: 2) 1.5 ml microfuge tube | Coolant: frozen gel packs | Days Tested:
M,W,F | Turnaround Time:
1-2 weeks | | Comments: |
Top of Page | Spring Viremia of Carp | | Fee: 25.00 | Specimen: 1) tissue
2) culture media
| Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested:
M-F | Turnaround Time:
3-5 days | | Comments: |
Top of Page | Transmissible Gastroenteritis Virus - TGEV | | Fee: 29.00 | Specimen: 1) feces
2) fecal swab
3) fresh small intestine
| Shipping Container: 1,3) whirl-pac or leakpoof container
2)swab | Coolant: frozen gel packs | Days Tested:
M,W,F | Turnaround Time:
3 days | | Comments: |
Top of Page | Viral Hemorrhagic Septicemia | | Fee: 25.00 | Specimen: 1) tissue
2) culture media
| Shipping Container: sterile container | Coolant: frozen gel packs | Days Tested:
M-F | Turnaround Time:
3-5 days | | Comments: RT-PCR or qRT-PCR |
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