VDL Identifies Pathogen Responsible for Several Mass Mortality Events of Wild Fish in MN
Beginning Tuesday November 14, 2017, the University of Minnesota Veterinary Diagnostic Laboratory will be offering a duplex PCR test for PCV2/PCV3. This test will replace the PCV2 test. The Universal PCV test offered by the UMN VDL detects PCV 1 and 2 (but will not differentiate between 1 and 2), while this new duplex detects PCV 2 and 3 (and differentiates between the two types).
Please see the factsheet for more information about this new test.
In July 2017, a type 2 North American strain of porcine reporoductive and respiratory syndrome (PRRSV-NA) was identified by indirect ELISA and RT-PCR in sows from 5 premises in Uruguay.
VDL faculty are among the authors of a longitudinal study on Senecavirus A shedding in the journal BMC Veterinary Research. The study found that while vesicular lesions had the highest concentration of virus, virus was detected on rectal and tonsil swabs for longer periods of up to six weeks.
Canine Influenza H3N2 was first identified in the United States in March of 2015 and spread to 23 states in 5 months. A second outbreak began in May 2017. Minnesota has had ten positive canine influenza cases in 2017 (as of 8/17/17) in Crow Wing, Kandiyohi, Ramsey, Sherburne and Wright counties. Although taken off the list of reportable diseases in 2016, canine influenza is back on the list of diseases reportable to the Board of Animal Health.
The Minnesota Board of Animal Health would like to remind clients that blood or serum samples submitted from animals in Minnesota for brucellosis testing for regulatory purposes must include the animal’s official identification on the submission form for each sample submitted. This includes samples from cattle, bison, cervids, and goats. Regulatory reasons for testing include but are not limited to herd certification, interstate or international movement.
Testing oral fluids for antibodies to Porcine Respiratory and Reproductive virus (PRRSv) is an efficient and effective way to monitor swine herds for exposure to this devastating virus. The VDL uses commercially manufactured kits for this testing.
In March 2017, Idexx reported that recent lots of PRRSV antibody test kit for oral fluids manufactured in the United States may have lower specificity producing occasional weak positives that run close to the assay cutoff (i.e. that do not confirm as true positives).
In order to recover costs, genome sequences (for i.e PRRSv, influenza A virus,etc) produced elsewhere and then submitted to the UMN VDL for dendrogram updates and/or comparative analysis to other case or vaccine strains, will incur a charge of $40 per sequence plus a case accession fee beginning March 1, 2017. There will be no fee for the analysis and dendrogram maintenance for any sequences performed at the U of MN VDL. For more information click here or contact the VDL.
An indirect ELISA assay was developed to specifically identify serum antibodies to Senecavalley virus A (SVA) by Dr. Michael Murtaugh’s Laboratory. The plates were coated with antigen prepared by purification of E. coli cloned and expressed viral protein 2 (VP2). To validate, positive serum samples were obtained from 34 sows clinically diagnosed with vesicular lesions and bled periodically over a 60-day period starting at the first observation of clinical signs (sampling at day 0, 4, 11,18, 25, 39, and 60; n=205).
- Change to Mycohyo PCR test
- Porcine Sapelo/Tescho virus Fact Sheet
- PCV 2/3 Fact Sheet
- Canine Influenza FAQs
- PRRSv Resistance in Gene-Altered Pigs
- Avoiding Heat Stress in Poultry
- Influenza A in Swine Testing Algorithm
- Poultry submisson video
- Tularemia testing information
- USDA Recommendation for swine with potential vesicular disease