Testing oral fluids for antibodies to Porcine Respiratory and Reproductive virus (PRRSv) is an efficient and effective way to monitor swine herds for exposure to this devastating virus. The VDL uses commercially manufactured kits for this testing.
In March 2017, Idexx reported that recent lots of PRRSV antibody test kit for oral fluids manufactured in the United States may have lower specificity producing occasional weak positives that run close to the assay cutoff (i.e. that do not confirm as true positives).
In order to recover costs, genome sequences (for i.e PRRSv, influenza A virus,etc) produced elsewhere and then submitted to the UMN VDL for dendrogram updates and/or comparative analysis to other case or vaccine strains, will incur a charge of $40 per sequence plus a case accession fee beginning March 1, 2017. There will be no fee for the analysis and dendrogram maintenance for any sequences performed at the U of MN VDL. For more information click here or contact the VDL.
An indirect ELISA assay was developed to specifically identify serum antibodies to Senecavalley virus A (SVA) by Dr. Michael Murtaugh’s Laboratory. The plates were coated with antigen prepared by purification of E. coli cloned and expressed viral protein 2 (VP2). To validate, positive serum samples were obtained from 34 sows clinically diagnosed with vesicular lesions and bled periodically over a 60-day period starting at the first observation of clinical signs (sampling at day 0, 4, 11,18, 25, 39, and 60; n=205).
Detection of a novel porcine circovirus – PCV3 – by UMN VDL diagnosticians and researchers and others was recently reported in the journal Virology. The paper describes tissue lesions in pigs that were associated with PCV3 infection.
Dr. Anibal Armién and Jan Shivers, VDL Histology section head, along with Dr. Stephanie Valberg and several other researchers from the University of Minnesota co-authored a publication on equine shivers which won the prestigious Davis-Thompson Foundation (former CL Davis Foundation) Journal award for best article in the journal Veterinary Pathology for the year 2016.
The Davis-Thompson Foundation is an international organization whose goal is the advancement of veterinary and comparative pathology.
Ileana Miranda, a former J1 scholar at the VDL working with Dr. Anibal Armién, won the ACVP Young Investigators Award Poster Competition in the category of Diagnostic Pathology. The poster entitled "Ultrastructure of Air-conducting Mucosa of Dogs with Chronic Respiratory Disease Suspected of Primary Ciliary Dyskinesia" was based on work she did at the VDL with Dr. Armién in 2015. Ileana completed her masters program in Rio de Janeiro, Brazil and has been accepted into a veterinary pathology residency at Cornell University.
Matthew Sturos DVM, UMN VDL pathologist and PhD student received the Charles Louis Davis DVM Foundation for the Advancement of Veterinary and Comparative Pathology 2016 Student Scholarship award in veterinary pathology at this years American College of Veterinary Pathologists' meeting in New Orleans December 2016. Congratulations to Matt!
On Monday, November 7th, 2016, The University of Minnesota Veterinary Diagnostic Laboratory (VDL) will begin offering a real time multiplex PCR that simultaneously detects Porcine Epidemic Diarrhea Virus (PEDV), Porcine Deltacoronavirus (PDCoV) and Porcine Transmissible Gastrointestinal Virus (TGEV) in porcine samples (individual oral fluids or environmental samples; individual or pooled intestinal homogenates, feces or fecal swabs). The assay will be tested Monday through Friday and routine results will be available within 24 working hours (or sooner if designated as rush/same day).
St. Paul, Minn. — The Minnesota Poultry Testing Laboratory (MPTL), located in Willmar, will re-open
after an extensive building expansion project that began last December. Serving farmers, poultry
producers and veterinarians in the area, the project will provide additional diagnostic testing labs and
enhanced equipment and office spaces.
The new construction expanded the 20-year-old space from a 3,665 square-foot building to an 11,890
square-foot one. MPTL is a collaboration between the University of Minnesota, College of Veterinary
Dean Ames congratulated Londa Kroone on her 40 year of service to the VDL during the Staff Appreciation Day ceremony on July 21, 2016.
Londa completed a Laboratory Assistant program at the University of Minnesota in 1967 and after working in several laboratories both within and outside the University, came to the VDL in 1976. She worked in the serology lab for her entire career. When she started she was responsible for just 4 tests. The number of tests done in her lab has jumped to more than 10 and include canine brucellosis, anaplasmosis and equine infectious anemia.
- Change to M. hyo PCR test
- Porcine Sapelo/Tescho virus Fact Sheet
- PCV 2/3 Fact Sheet
- Canine Influenza FAQs
- PRRSv Resistance in Gene-Altered Pigs
- Avoiding Heat Stress in Poultry
- Influenza A in Swine Testing Algorithm
- Poultry submisson video
- Tularemia testing information
- USDA Recommendation for swine with potential vesicular disease