UMN VDL now offering Parachek 2 ELISA for detection of antibodies to Johne's disease in sheep and goats

May 2, 2016

The Serology laboratory at MVDL will start offering Parachek 2 ELISA for detection of antibodies against Mycobacterium paratuberculosis (Johne’s disease) in serum samples of sheep and goats. The test kit is imported from Europe and was recently validated in our lab. Results of 45 serum samples were compared with those obtained from Johne’s Information Center at University of Wisconsin, School of Veterinary Medicine at Madison, WI. There was 89% agreement between the two labs (Kappa=0.77). Analysis of results on samples tested in duplicate revealed 96% agreement (Kappa=0.90). This test will replace Mycobacterium paratuberculosis Biocor ELISA which is currently unavailable.  Results for samples from other species e.g. deer, elk, moose etc. will be reported with a disclaimer.