Next Generation Sequencing Disease Investigation - RNA
1) At least 400 uL (0.4 mL) 2) at least 2 g tissue
For disease investigation when RNA viruses are suspected. Some DNA viruses may be identified as well, but are not the focus of this procedure. If investigation of both RNA and DNA pathogens is desired, please consider our Metagenomic service.
We use Illumina technology for sequencing. We cannot guarantee the success of NGS procedures as this technique is the unbiased amplification of all nucleic acids in the sample. The number of reads for a pathogen of interest may not be sufficient to produce a full genome. In those instances, a draft genome may be produced and additional sequencing would be necessary to fill in gaps in the genome. Additional sequencing options and costs will be discussed if needed. When sufficient reads are available, full or draft genomes will be reported with annotations, comparison with vaccine or reference sequences, recombinant/reassortment analysis, and dendrograms.