Bacteriology/Mycology

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Submission form


One of the newest tools used by the Bactiology lab is the MALDI-TOF spectrometer.

Watch avideo about the UMN VDL MALDI-TOF and Sensititre:

The Bacteriology Section of the VDL cultures a wide variety of bacteria that can cause disease in animals. The lab can also conduct sensitivity tests to determine the suseptibility of the bacteria to specific antibiotics. In addition, the VDL can conduct PCR, ELISA, serotyping and genetic sequencing on specific types of bacteria. 

Services and Fees

Effective August 31, 2015

Please use the Necropsy and Sample Submission form.

Note: A $10 accession fee will be applied to all submissions. All fees are in US $.

Note on days tested and turnaround times: Samples must be received at least one business day before the scheduled testing day. New client accounts can take up to 2-3 business days to establish before testing can begin. Samples may not always be run on the first scheduled day after they arrive; however, it is our goal to complete testing within the posted turnaround time. If rush testing is desired, please contact us.

Click the name of the test or procedure for detailed information. Tests marked with an asterisk (*) will redirect to the Bacterial PCR page.

Tests marked with a double asterisk (**) have the following disclaimer: Poultry Salmonella Testing is for BAH/NPIP approved programs only.

Specimen Requirements:

1) affected tissue 2) smear or slide 3) swab

Shipping Container:

1) sterile container 2) slide folder 3) culturette

Coolant:
1,3) frozen gel packs 2) none
Fee:
10.25
Days Tested:
M-F
Turnaround Time:
1 business day
Comments:
 
 
Specimen Requirements:

1) swab 2) affected tissue

Shipping Container:

1) culturette 2) sterile container

Coolant:
frozen gel packs
Fee:
21.50
Days Tested:
M-F
Turnaround Time:
2-5 business days
Comments:

Samples should be kept cool and moist in transit.

Specimen Requirements:

1) swab 2) affected tissue

Shipping Container:

1) anaerobic transport media 2) sterile container

Coolant:
1) none 2) frozen gel packs
Fee:
23.25
Days Tested:
M-F
Turnaround Time:
2-5 business days
Comments:

Ship overnight.

Specimen Requirements:

live fish

Shipping Container:

leakproof container

Coolant:
none
Fee:
94.50 per lot (up to 60 fish)
Days Tested:
M-W
Turnaround Time:
10 business days
Comments:

See the Aquaculture/Fisheries Submission Form for details on tests and pathogens. Does not include Renibacterium salmoninarum IFAT.

Specimen Requirements:

isolate

Shipping Container:
Coolant:
Fee:
93.00
Days Tested:
Turnaround Time:
Comments:
Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
20.00
Days Tested:
M-F
Turnaround Time:
7 business days
Comments:

Prevent agar from freezing.

Specimen Requirements:

5 ml blood

Shipping Container:

100 ml blood culture bottles

Coolant:
none
Fee:
26.25
Days Tested:
M-F
Turnaround Time:
2-8 business days
Comments:
Specimen Requirements:

5 ml blood

Shipping Container:

100 ml blood culture bottles

Coolant:
none
Fee:
29.00
Days Tested:
M-F
Turnaround Time:
3-10 business days
Comments:
Specimen Requirements:

1) tissue 2) swab 3) pure culture

Shipping Container:

1) sterile container 2) culturette 3) agar plate in anaerobic bag

Coolant:
1,2) frozen gel packs 3) none
Fee:
50.00
Days Tested:
needs to be scheduled by batches
Turnaround Time:
2 weeks
Comments:

Please call to schedule. Currently just for Carbadox; other antimicrobial agar dilution MICs may be performed as needed.

Specimen Requirements:

1) tissue 2) swab 3) pure culture

Shipping Container:

1) sterile container 2) culturette 3) agar plate in anaerobic bag

Coolant:
1,2) frozen gel packs 3) none
Fee:
60.00
Days Tested:
as needed
Turnaround Time:
2 weeks
Comments:

Panel includes Tiamulin, Valnemulin, Doxycycline, Tylvalosin, Lincomycin, and Tylosin

Specimen Requirements:

1) fresh colon section 2) fresh feces

Shipping Container:

1,2) sterile container

Coolant:
frozen gel packs
Fee:
26.25
Days Tested:
M-F
Turnaround Time:
10-14 business days
Comments:

Ship overnight or deliver the same day.

Specimen Requirements:

1) aborted fetus 2) prepucial flush 3) cervical mucous

Shipping Container:

1) sterile container 2,3) campy transport media

Coolant:
frozen gel packs
Fee:
34.75
Days Tested:
M-F
Turnaround Time:
6-14 business days
Comments:

Transport media is supplied by the laboratory.

Specimen Requirements:

1) feces 2) intestine (tied loop)

Shipping Container:

1,2) sterile container

Coolant:
frozen gel packs
Fee:
34.75
Days Tested:
M-F
Turnaround Time:
6-14 business days
Comments:

Use anaerobic transport media if delayed more than 24 hours.

Specimen Requirements:

swabs

Shipping Container:

plastic tube

Coolant:
frozen gel packs
Fee:
30.25
Days Tested:
M-F
Turnaround Time:
2-3 business days
Comments:
Specimen Requirements:

feces in Chlamydia transport media

Shipping Container:

sealed container

Coolant:
frozen gel packs
Fee:
37.25
Days Tested:
Thursday
Turnaround Time:
2-3 weeks
Comments:

Contact lab for transport media.

Specimen Requirements:

1) 1 gm intestinal contents, or 2) 1 gm of feces

Shipping Container:

1,2) plastic bag or tube

Coolant:
frozen gel packs
Fee:
16.50
Days Tested:
once weekly
Turnaround Time:
2-7 business days
Comments:

Tests run when sufficient number of samples are available.

Specimen Requirements:

swab

Shipping Container:

culturette

Coolant:
frozen gel packs
Fee:
32.75
Days Tested:
M-F
Turnaround Time:
2-5 business days
Comments:

Samples should be kept cool and moist in transit.

Specimen Requirements:

1) affected tissue 2) 5 thin smears

Shipping Container:

1) sterile container 2) slide folder

Coolant:
1) frozen gel packs 2) none
Fee:
15.25
Days Tested:
M-F
Turnaround Time:
1 business day
Comments:

Fix slides in acetone for five minutes.

Specimen Requirements:

1) affected tissue 2) hair or scraping 3) swab

Shipping Container:

1) sterile container 2) envelope 3) culturette

Coolant:
1,3) frozen gel packs 2) none
Fee:
21.75
Days Tested:
M-F
Turnaround Time:
4-6 weeks
Comments:
Specimen Requirements:

1) affected tissue 2) smear or slide 3) swab 4) hair sample

Shipping Container:

1) sterile container 2) slide folder 3) culturette 4) envelope

Coolant:
1,3) frozen gel packs 2,4) none
Fee:
14.75
Days Tested:
M-F
Turnaround Time:
1 business day
Comments:
Specimen Requirements:

1) pure culture 2) impure culture

Shipping Container:

1,2) agar plate or slant

Coolant:
none
Fee:
1) 34.75 2) 47.50
Days Tested:
M-F
Turnaround Time:
5 business days
Comments:

Prevent agar from freezing.

Specimen Requirements:

1) ileum 2) feces

Shipping Container:

1,2) sterile container

Coolant:
frozen gel packs
Fee:
32.50
Days Tested:
M-F
Turnaround Time:
7 bus. days
Comments:
Specimen Requirements:

1) ileum 2) feces

Shipping Container:

1,2) sterile container

Coolant:
frozen gel packs
Fee:
32.50
Days Tested:
M-F
Turnaround Time:
7 business days
Comments:
Specimen Requirements:

1) tissue 2) silage

Shipping Container:

1) sterile container 2) clean, sealed container

Coolant:
1) frozen gel packs 2) none
Fee:
23.75
Days Tested:
M-F
Turnaround Time:
4-30 business days
Comments:
Specimen Requirements:

feces (no more than 10g needed)

Shipping Container:

Do NOT use GLOVE or WHIRL-PAC BAG as container. Use leakproof hard plastic container with screw cap

Coolant:
frozen gel packs or dry ice
Fee:
21.75
Days Tested:
T
Turnaround Time:
3-7 business days
Comments:

Please make sure container has no outside contamination. NO GLOVES or WHIRL PAC BAGS. 
Specimens from sheep, goat, and exotic ruminants also accepted.

Ct Values from this assay correspond to the shedding status of the animal as follows:
Less than Ct 25 = High Shedding
Ct 25 to Ct 30 = Moderate Shedding
Ct 30 to Ct 37 = Low Shedding
Ct 37 to Ct 40 = Suspect Animal
Above 40 or no Ct value = Negative

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

The lab may pool specimens with up to 5 samples per pool - please indicate on the submission form. Pooling results in slightly decreased test sensitivity. 
DO NOT PRE-POOL SAMPLES.

Samples must be submitted by Tuesday and results are reported on Thursdays.

 

Specimen Requirements:

1) bronchial swab or lavage 2) lung tissue

Shipping Container:

1) plastic tube 2) sterile container

Coolant:
frozen gel packs
Fee:
105.00
Days Tested:
as needed
Turnaround Time:
4-5 weeks
Comments:

Please call to schedule. More information.

Specimen Requirements:

1) uterine, cervical, or vaginal mucus 2) semen 3) lung tissue 4) tracheal wash 5) fluid

Shipping Container:

1,2,4,5) Amies transport media 3) sterile container

Coolant:
frozen gel packs
Fee:
30.00
Days Tested:
M-F
Turnaround Time:
14 business days
Comments:

Ship overnight or deliver the same business day.

Specimen Requirements:

1) tissue 2) feces

Shipping Container:

1,2) clean container

Coolant:
frozen gel packs
Fee:
21.50
Days Tested:
M-F
Turnaround Time:
2-5 business days
Comments:
Specimen Requirements:

1) environmental samples 2) cloacal swab 3) whole bird

Shipping Container:

1) 18 oz. specimen bag 2) lab provided 3) clean container

Coolant:
frozen gel packs and cooler
Fee:
25.75
Days Tested:
M-W
Turnaround Time:
4 weeks
Comments:

MPTL

Specimen Requirements:

1) environmental samples 2) cloacal swab 3) whole bird

Shipping Container:

1) 18 oz. specimen bag 2) lab provided 3) clean container

Coolant:
frozen gel packs and cooler
Fee:
22.00
Days Tested:
M-W
Turnaround Time:
4 weeks
Comments:

MPTL

Specimen Requirements:

culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
11.00
Days Tested:
M-Th
Turnaround Time:
4 weeks
Comments:

MPTL

Specimen Requirements:

1) chicks 2) feed 3) environmental samples

Shipping Container:

1,2,3) clean container (well-labeled)

Coolant:
1) frozen gel packs 2,3) none
Fee:
25.75
Days Tested:
M-F
Turnaround Time:
5-14 business days
Comments:
Specimen Requirements:

eggs

Shipping Container:

clean container (well labeled)

Coolant:
none
Fee:
29.00/pool of 20
Days Tested:
M-F
Turnaround Time:
5-7 business days
Comments:

Prevent eggs from freezing or breaking. Submission of extra eggs is recommended to account for breakage during shipment.

Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
8.00
Days Tested:
M-F
Turnaround Time:
1-2 business days
Comments:

Prevent agar from freezing

Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
8.00
Days Tested:
M-F
Turnaround Time:
4 weeks
Comments:

MPTL

Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
no charge for diagnostic isolates (see comments)
Days Tested:
M-F
Turnaround Time:
4-6 weeks
Comments:

$40.00 charge for research, export and environmental isolates.Prevent agar from freezing.

Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
13.25
Days Tested:
M-F
Turnaround Time:
4-6 weeks
Comments:

MPTL

Specimen Requirements:

pure culture

Shipping Container:

agar plate or slant

Coolant:
none
Fee:
ref. lab fee
Days Tested:
M-F
Turnaround Time:
2-3 weeks
Comments:

Prevent agar from freezing.

Specimen Requirements:

1) tissue 2) swab 3) pure culture

Shipping Container:

1) sterile container 2) culturette 3) agar plate or slant

Coolant:
1,2) frozen gel packs 3) none
Fee:
19.00
Days Tested:
M-F
Turnaround Time:
2-4 business days
Comments:
Specimen Requirements:

1) prepucial flush 2) cervical mucus 3) 1 gram feces*

Shipping Container:

1,2) Trichomonas transport media 3) leak proof container

Coolant:
none
Fee:
18.50
Days Tested:
M-F
Turnaround Time:
4 business days
Comments:

Transport media is furnished by the lab. Additional information. *3) Feline only. FRESH feces kept at room temp. At least a kidney bean size sample.

Specimen Requirements:

1) uterine, cervical or vaginal mucus 2) semen 3) lung tissue 4) tracheal wash 5) fluid

Shipping Container:

1,2,4,5) Amies transport media 3) sterile container

Coolant:
frozen gel packs
Fee:
26.25
Days Tested:
M-F
Turnaround Time:
7 business days
Comments:

Ship overnight or deliver the same day.

Specimen Requirements:

urolith

Shipping Container:

clean container

Coolant:
none
Fee:
71.50
Days Tested:
M-F
Turnaround Time:
3-4 business days
Comments:
Specimen Requirements:

1) feces 2) tissue

Shipping Container:

1,2) sterile container

Coolant:
frozen gel packs
Fee:
26.25
Days Tested:
M-F
Turnaround Time:
2-4 business days
Comments: