Negative image of an Adenovirus (A), Calicivirus (B), Parvovirus (C), Coronavirus (D), Parapoxvirus (E), Orthopoxvirus (F), Rotavirus (white arrow), Enterovirus (black arrow) (G) and Influenza virus (H). Transmission electron micrographs - viruses recovered from feces, cell culture and tissue samples, negative stain with 1% PTA.
Iridovirus infection in Banggai cardinalfish. A) Necrotizing nephritis with intra-cytoplasmic basophilic inclusion bodies (arrows), paraffin-embedded, 5 µm section stained with H&E. B) Symmetric aggregation of 250 nm hexagonal virions. Electron microscopy preparation obtained from paraffin-embedded tissue that was fixed in formaldehyde approximately 4 to 8 hours after death.
Systemic mycobacteriosis in a toad. A) Macrophage with intracellular Mycobacterium marinum in a phagolysosome (Pl), nucleus (N), mitochondrion (M). B) Phagolysosome with numerous bacilli (arrows); phagolysosome membrane (Plm). Postmortem sample fixed in formaldehyde approximately 15 to 20 hours after death.
Gastric cryptosporidiosis in a snake. A) H&E-stained section with dilated gland lined by extra- and intracellular forms of Cryptosporidium sp. B) Electron micrographs from gastric gland showing Meront (m) , within an epithelial cell (E) and oocysts (O) free in the gland’s lumen, glutaraldehyde-fixed sample collected approximately 3 hours after euthanasia. Inset: Oocyst recovered from feces. Feces preserved in potassium dichromate, oocysts concentrated, plastic- embedded and stained with lead citrate/uranyl acetate.
Renal coccidiosis in a bat. A) Dilated renal tubule with intracellular intermediary forms of the protozoan. 1µm Toluidine blue-stained section, sample fixed within 15 minutes after euthanasia; B) Negative image of the apical cytoskeleton showing conoid subunit (C ) and microtubules (m). Direct smear of cyst content stained negatively with 1% PTA.
Malignant melanoma in a skink. A) Neoplastic melanocytes with abnormal mature (arrows) and immature melanosome, nucleus (N). B) Melanocyte from the control skin section of a skink, mature melanosome (arrows). Postmortem sample fixed in formaldehyde approximately 3 to 4 hours after death.
Ciliary dyskinesia in a dog. A) Nasal turbinate with severe catarrhal suppurative inflammation. Inset: Chronic lympho-plasmacytic rhinitis, H&E-stained section from A. B) Electron micrograph of nasal epithelium, cilia defects characterized by absence of outer and inner dynein arms, disorganization of peripheral doublets, central doubles and supernumerary singles (arrows). In general abnormal microtubule arrays were composed of 6+1 to 6+4, 7+1 to 7-4, 8+1 to 8+3, and 9+0 to 9+2. Glutaraldehyde-fixed sample collected approximately 30 minutes after euthanasia.
Type I equine polysaccharide storage myopathy . A) Subsarcolemmal accumulation β-glycogen particles. b, c) β-glycogen particles dissecting myofibrils. Electron microscopy preparation obtained from paraffin-embedded tissue that was fixed in formaldehyde within 15 minutes after biopsied.