Viral PCR

Services & Fees

Effective  June 15, 2018

Note: A $10 accession fee will be applied to all submissions. A centrifugation charge of $1.25 per tube may be applied. All fees are in US $.

Note on days tested and turnaround times: Samples must be received at least 1 business day before the scheduled testing day. New client accounts can take up to 2-3 business days to establish before testing can begin. Samples may not always be tested on the first scheduled day after they arrive; however, it is our goal to complete testing within the posted turnaround time. If rush testing is desired, please contact us.

Pooling any sample may produce a false negative result. Pooling strategies should consider the characteristics/pathogenesis of the disease and test being used.

Submission form

Click the name of the test or procedure for detailed information:

Specimen Requirements:

tracheal swabs

Shipping Container:

15 ml screw cap vial (5 swabs/vial)

Coolant:
frozen gel packs
Fee:
26.25
Days Tested:
T
Turnaround Time:
7 bus. days
Comments:

10 swabs used per test. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. This test is conducted at the Minnesota Poultry Testing Laboratory location only.  

Specimen Requirements:

1) lung, intestine or other tissue homogenate 2) feces 3) fecal or rectal swabs 4) nasal swabs

Shipping Container:

1) leakproof 1.5 ml microfugetube 2) whirl pac or plastic bag 3,4) synthetic swabs

Coolant:
frozen gel pack
Fee:
34.00
Days Tested:
M,Th
Turnaround Time:
5 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) lung 2) virus isolate 3) nasal ortracheal swabs

Shipping Container:

1) whirl pac or plastic bag 2) leakproof 1.5 ml microfuge tube 3) synthetic swabs

Coolant:
frozen gel packs , if not frozen previously. If already frozen, send on dry ice
Fee:
25.00
Days Tested:
W
Turnaround Time:
5 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) feces 2) intestine 3) feedback material 4) environmental swabs 5) virus isolate

Shipping Container:

1,2,3) whirl-pac or leakpoof container 4) swab 5) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
28.25
Days Tested:
M,Th
Turnaround Time:
7 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) PCR positive sample 2) Virus isolate

Shipping Container:

2) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
156.50
Days Tested:
Weekly (as needed)
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) serum (at least 0.5 ml) 2) tissue

Shipping Container:

1) leakproof 1.5 ml microfuge tube 2) whirl pac or plastic bag

Coolant:
frozen gel packs
Fee:
1) 7.00 2) 18.00
Days Tested:
T,Th
Turnaround Time:
5 business days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) serum 2) tissue 3) buffy coat

Shipping Container:

1) leakproof 1.5 ml microfuge tube 2) whirl pac or plastic bag 3) 3-5 ml purple top whole blood tube

Coolant:
frozen gel packs
Fee:
7.00 (see comments)
Days Tested:
T,Th
Turnaround Time:
5 business days
Comments:

Cost is per sample. Samples will be pooled in groups up to 5 and results will be reported as individual animals. 3) Processing of whole blood samples requires extra time, and could add 1-2 days to the turnaround time of the assay. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1cm x 1cm ear tissue (1/2 inch notching pliers)

Shipping Container:

6 or 10 ml red top tube (no additive or gel)

Coolant:
frozen gel packs
Fee:
33.00
Days Tested:
T,Th
Turnaround Time:
5 business days
Comments:

Please follow these Submission Guidelines.  Samples will be run in pools up to 50. See Virology for BVD ear notch individual testing. Processing of ear notch samples requires extra time, and could add 1-2 days to the turnaround time of the assay. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) serum (at least 300 uL) 2) virus isolate (at least 300 uL) 3) tissue (1 cm3 or biopsy)

Shipping Container:

1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag

Coolant:
frozen gel packs
Fee:
159.00
Days Tested:
weekly
Turnaround Time:
2-4 weeks
Comments:

2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery. 

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

nasal or pharyngeal swabs

Shipping Container:

synthetic swabs

Coolant:
frozen gel packs
Fee:
25.00
Days Tested:
M-F
Turnaround Time:
2-3 days
Comments:

Shipping instruction; send next day delivery.The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) serum (at least 300 uL)   2) virus isolate (at least 300 uL)   3) tissue (1 cm3 or biopsy)   4) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) 1.5 ml microfuge tube  3) whirl-pac or plastic bag  4) sterile tube or whirl pac

Coolant:
frozen gel packs
Fee:
130.00
Days Tested:
weekly
Turnaround Time:
2-4 weeks
Comments:

2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples. 

When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) semen (at least 0.5 ml) 2) serum (at least 0.5 ml) 3) tissue 4) blood swabs 5) feces 6) oral fluids/saliva (at least 2 ml)   7) Processing fluids

Shipping Container:

1,4) leakproof 1.5 ml microfuge tube 2) red top tube 3,5) whirl-pac or plastic bag 6) sterile tube or whirl pac 7) 10,15, or 50 ml plastic tubes with screwtop caps

Coolant:
frozen gel packs
Fee:
30.00
Days Tested:
T,F
Turnaround Time:
5 business days
Comments:

6) Do not pool group/pen samples. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) serum (at least 0.5 ml) 2) tissue 3) feces

Shipping Container:

1) leakproof 1.5 ml microfuge tube 2,3) whirl-pac or plastic bag

Coolant:
frozen gel packs
Fee:
46.75
Days Tested:
T,F
Turnaround Time:
5 business days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) tissue 2) virus isolate

Shipping Container:

1) whirl-pac or plastic bag 2) leakproof 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
34.00
Days Tested:
Thursday
Turnaround Time:
7 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

brain tissue

Shipping Container:
Coolant:
Fee:
no charge
Days Tested:
daily
Turnaround Time:
4 bus. days
Comments:

Sent to Minnesota Dept. of Health.

Specimen Requirements:

1) Nasal swabs 2) Whole blood

Shipping Container:

Styrofoam box

Coolant:
Cold packs
Fee:
ref. lab fee
Days Tested:
Turnaround Time:
Comments:

This test is performed at University of California-Davis. Please contact them for testing information 1-530-752-8700 .

Specimen Requirements:

tracheal swabs

Shipping Container:
Coolant:
frozen gel packs
Fee:
41.00
Days Tested:
Weekly
Turnaround Time:
7 business days
Comments:

Swabs may be pooled up to 5. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. Routine END testing is done at the Minnesota Poultry Testing Laboratory.

Specimen Requirements:

Please email external sequences to vdladmin@umn.edu and the pathologist assigned to the case. 

Shipping Container:

N/A

Coolant:
N/A
Fee:
$40.00 plus accession fee
Days Tested:
as needed
Turnaround Time:
2 business days
Comments:

There will be no additional fee for the analysis and dendrogram maintenance for any sequences performed at the U of MN VDL.

H1 clades are determined using the Swine H1 Clade Classification available from the NIAID Influenza Research Database (IRD) [Zhang Y, et al. (2017)] through the web site at http://www.fludb.org.    For more information on H1 clade information and methodology, please refer to "A Phylogeny-Based Global Nomenclature System and Automated Annotation Tool for H1 Hemagglutinin Genes from Swine Influenza A Viruses." Tavis K. Anderson, Catherine A. Macken, et.al, mSphere Dec 2016, 1 (6) e00275-16; DOI: 10.1128/mSphere.00275-16.

 H3 clades are determined using a public data set of Swine H3 Clade Classification reference strains and the BLASTN 2.2.22 tool available from the NIAID Influenza Research Database (IRD) [Zhang Y, et al. (2017)] through the website at http://www.fludb.org.   For more information regarding Swine H3 clade classifications, please refer to "Sequences used in swine H3 clade classification reference tree." Tavis K. Anderson, Catherine A. Macken, et. al. Virus Pathogen Database and Analysis Resource (ViPR) -. N.p., 03 Nov. 2016. Web. 21 Feb. 2017.

 For a review of influenza in pigs in the USA, read this lay publication on this website: https://www.pig333.com/swine-influenza/the-genetic- diversity-of- contemporary-swine- influenza-a- viruses-in- usa_11593/

Specimen Requirements:

1) tissue 2) virus isolate 3) oral fluids

Shipping Container:

1) whirl-pac or plastic bag 2,3) leakproof 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
156.00
Days Tested:
as needed
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) tissue 2) virus isolate 3) oral fluids

Shipping Container:

1) whirl-pac or plastic bag 2,3) leakproof 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
34.00
Days Tested:
M,Th
Turnaround Time:
5 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) swabs (nasal, conjunctival, vaginal or preputial) 2) lung or other tissue 3) virus isolate 4) tracheal wash or lavage

Shipping Container:

1) synthetic swabs 2) whirl-pac or plastic bag 3) leakproof 1.5 ml microfuge tube 4) sterile tube

Coolant:
1,2,4) frozen gel packs 3) dry ice
Fee:
25.00
Days Tested:
W
Turnaround Time:
5 business days
Comments:

Results reported on Wednesdays (samples must be received at least the day before test date). The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) nasal or pharyngeal swabs   2) tracheal or oropharyngeal swabs 3) virus isolate (at least 0.5 ml)   4) lung (2-3 cm3 or biopsy)   5) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) synthetic swabs 3) leakproof 1.5 ml microfuge tube 4) whirl-pac or plastic bag

Coolant:
1,3)frozen gel packs 2) dry ice
Fee:
23.00
Days Tested:
T
Turnaround Time:
1-2 weeks
Comments:

Subtyping tests are run on samples previously testing positive for influenza virus.
2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples.

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

swabs

Shipping Container:
Coolant:
Frozen gel packs
Fee:
16.00
Days Tested:
As needed on positive Matrtix results
Turnaround Time:
24 hrs. (from test date)
Comments:

Testing for subtyping is run after a positive is obtained by Influenza virus Matrix testing. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. Routine avian influenza testing is done at the Minnesota Poultry Testing Laboratory.

Specimen Requirements:

1) nasal or pharyngeal swabs 2) tracheal or oropharyngeal swabs (avian) 3) lung (2-3 cm3 or biopsy) 4) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) synthetic swabs 3,4) whirl-pac or plastic bag

Coolant:
frozen gel packs
Fee:
30.00
Days Tested:
M-F
Turnaround Time:
2-3 days
Comments:

Shipping instruction: send next day delivery.

For avian, swabs may be pooled up to 5. Routine avian testing is done at the Minnesota Poultry Testing Laboratory. 

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) nasal swabs   2) viral isolate (at least 0.5 ml)   3) lung (2-3 cm3 or biopsy)   4) oral fluids/saliva (at least 2 ml)

Shipping Container:

1) synthetic swabs  2) leakproof 1.5 ml microfuge tube  3,4) whirl-pac or plastic bag

Coolant:
1,3,4) frozen gel packs 2) dry ice
Fee:
149.00
Days Tested:
Tuesday
Turnaround Time:
2-4 weeks
Comments:

2) Must come with a copy of an USDA shipping permit.
3) Shipping instruction: send next day delivery.
4) Do not pool group/pen samples. 

When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

H1 clades are determined using the Swine H1 Clade Classification available from the NIAID Influenza Research Database (IRD) [Zhang Y, et al. (2017)] through the web site at http://www.fludb.org.    For more information on H1 clade information and methodology, please refer to "A Phylogeny-Based Global Nomenclature System and Automated Annotation Tool for H1 Hemagglutinin Genes from Swine Influenza A Viruses." Tavis K. Anderson, Catherine A. Macken, et.al, mSphere Dec 2016, 1 (6) e00275-16; DOI: 10.1128/mSphere.00275-16.

 H3 clades are determined using a public data set of Swine H3 Clade Classification reference strains and the BLASTN 2.2.22 tool available from the NIAID Influenza Research Database (IRD) [Zhang Y, et al. (2017)] through the website at http://www.fludb.org.   For more information regarding Swine H3 clade classifications, please refer to "Sequences used in swine H3 clade classification reference tree." Tavis K. Anderson, Catherine A. Macken, et. al. Virus Pathogen Database and Analysis Resource (ViPR) -. N.p., 03 Nov. 2016. Web. 21 Feb. 2017.

For a review of influenza in pigs in the USA, read this lay publication on this website: https://www.pig333.com/swine-influenza/the-genetic- diversity-of- contemporary-swine- influenza-a- viruses-in- usa_11593/

Specimen Requirements:

1) tissue 2) culture media

Shipping Container:

sterile container

Coolant:
frozen gel packs
Fee:
27.50
Days Tested:
as needed
Turnaround Time:
3-5 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

tracheal swabs

Shipping Container:
Coolant:
frozen gel packs
Fee:
41.50
Days Tested:
W
Turnaround Time:
1-2 bus. days (from test date)
Comments:

Swabs may be pooled up to 5. Fusion PCR is run to detect virulent NDV after a positive is obtained by END virus Matrix PCR testing. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) nasal turbinates 2) nasal swabs 3) buffy coat

Shipping Container:
Coolant:
frozen gel packs
Fee:
65.00
Days Tested:
First Tuesday of the month
Turnaround Time:
One month
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) feces 2) fecal swab 3) fresh small intestine 4) viral isolate

Shipping Container:

1,3) whirl-pac or leakproof container 2) swab

Coolant:
frozen gel packs
Fee:
350.00
Days Tested:
Thursday
Turnaround Time:
2-4 weeks
Comments:

For optimal result, sequencing should only be requested on highly positive samples with Ct values of 25 or less. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

This procedure is validated, but is not performed within the VDL quality system.

Specimen Requirements:

1) Intestines (at least 1 cm3);  2) oral fluid (at least 2 ml);  3) feces;  4) Fecal swabs;  5) Environmental swabs   

Shipping Container:

1,3) whirl-pac or plastic bag;  2) tube or plastic bag;   4,5) swab

Coolant:
Frozen gel packs
Fee:
31.00
Days Tested:
F
Turnaround Time:
5 Business Days
Comments:

Comments:

This qPCR will distinguish between PEDV virulent and variant strains. The variant strain is the clinically less severe form of the disease. Prior screening of samples to ensure PEDV status is recommended. 

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Fact Sheet on PEDV Differentiation Test

Specimen Requirements:

1) feces  2) fecal swab  3) fresh small intestine  4) oral fluids  5) environmental swab

Shipping Container:

1,3,5) whirl-pac or leakproof container 2) swab 4) sterile tube

Coolant:
frozen gel packs
Fee:
31.50
Days Tested:
M-F
Turnaround Time:
24 business hours*
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

* Same day (boar stud monitoring) or next bus. day (regular request).   Holiday schedule for boar stud monitoring.

Specimen Requirements:

1) semen (at least 0.5 ml) 2) serum (at least 0.5 ml) 3) buffy coat 4) tissue 5) blood swabs 6) feces 7) oral fluids

Shipping Container:

1,2,5) leakproof 1.5 ml microfuge tube or serum separator tube 3) 3-5 ml purple top whole blood tube 4,6) whirl-pac or plastic bag 7) sterile tube

Coolant:
frozen gel packs
Fee:
34.00
Days Tested:
T
Turnaround Time:
5 business days
Comments:

A maximum of 5 samples/test will be pooled for serum, semen, or blood swab testing. The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) semen (at least 1 ml)   2) serum (at least 0.5 ml)   3) tissue (1 cm3 or biopsy)   4) blood swabs   5) nasal swabs   6) oral fluids/saliva (at least 2 ml) 7) Processing fluids

Shipping Container:

1,2,4) leakproof, 1.5 ml microfuge tube 3) whirl-pac or plastic bag 5) synthetic swabs 6) sterile tube or whirl pac 7) 10,15, or 50 ml plastic tubes with screw-top caps

Coolant:
frozen gel packs
Fee:
29.50
Days Tested:
M-F
Turnaround Time:
24 business hours*
Comments:

3) Shipping instruction: send next day delivery.
6) Do not pool group/pen samples.
The Veterinary Diagnostic Laboratory prefers pooling samples at the lab. Additional information.

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

*Same day (boar stud monitoring) or next bus. day (regular regular request).  Holiday schedule for boar stud monitoring.

Specimen Requirements:

1) serum (at least 300 uL)  2) virus isolate (at least 300 uL)  3) tissue (1 cm3 or biopsy)  4) extracted RNA  5) oral fluids/saliva (at least 2 ml)

Shipping Container:

1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag 4) sterile tube 5) sterile tube or whirl pac

Coolant:
frozen gel packs
Fee:
100.00
Days Tested:
M-F
Turnaround Time:
2-5 business days. Testing maybe longer on some samples
Comments:

To ensure that samples are added to the daily PRRS sequencing run, please see that the lab has the specific request by 8 am on the day of testing.

2) Must come with a copy of a USDA shipping permit.
3) Shipping instruction: send next day delivery.
5) Do not pool group/pen samples.
Additional information

When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) semen (at least 1 ml)  2) serum (at least 0.5 ml)  3) tissue (1 cm3 or biopsy)

Shipping Container:

1,2) 1.5 ml microfuge tube 3) whirl-pac or plastic bag

Coolant:
frozen gel packs
Fee:
34.00
Days Tested:
T
Turnaround Time:
5 business days
Comments:

3) Shipping instruction: send next day delivery. Additional information

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) semen (at least 1 ml)  2) serum (at least 0.5 ml)  3) tissue  4) blood swabs

Shipping Container:

1,2,4) leakproof, 1.5 ml microfuge tube 3) whirl-pac or plastic bag

Coolant:
frozen gel packs
Fee:
51.00
Days Tested:
As needed
Turnaround Time:
Comments:

3) Shipping instruction: send next day delivery. Additional Information.   The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) feces 2) intestine 3) feedback material 4) environmental swabs 5) virus isolate

Shipping Container:

1,2,3) whirl-pac or leakpoof container 4) swab 5) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
28.25
Days Tested:
T,Th
Turnaround Time:
3 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) PCR positive sample 2) virus isolate

Shipping Container:

2) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
156.50
Days Tested:
Weekly
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) PCR positive sample 2) Virus isolate

Shipping Container:

2) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
144.00
Days Tested:
Weekly
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) PCR positive sample 2) Virus isolate

Shipping Container:

2) 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
144.00
Days Tested:
Weekly
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) Spinal Cord; 2) brain; 3) tissue; 4) feces; 5) serum (at least 0.5 ml))

Shipping Container:

1,2,3,4) whirl-pac or plastic bag; 5) red top tube

Coolant:
Frozen gel packs
Fee:
$45
Days Tested:
Friday
Turnaround Time:
5 business days
Comments:

The specimen types listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested on fecal samples, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) Feces  2) Fecal swabs  3) Oral fluids  4) Serum  5) Blood swabs  6) Semen  7) Post-mortem tissues

Shipping Container:

1,7) whirl pac or plastic double bagged  2) synthetic swabs  3,6) sterile tube or whirl pac  4, 5) leakproof, 1.5 ml microfuge tube

Coolant:
frozen gel packs
Fee:
30.00
Days Tested:
Wednesday
Turnaround Time:
1 week
Comments:

Note:  Please place vesicle/ulcer swabs in 1 ml of sterile PBS or sterile saline.  Please keep samples chilled after collection and during shipment.

Specimen Requirements:

1) tissue 2) culture media

Shipping Container:

sterile container

Coolant:
frozen gel packs
Fee:
30.50
Days Tested:
as needed
Turnaround Time:
3-5 bus. days
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.

Specimen Requirements:

1) Feces  2) Fecal swabs  3) Fresh small intestine  4) Fresh liver tissue

Shipping Container:

1) Whirl pac or plastic bag  2) Synthetic swabs  3) Leak proof container  4) Leak proof container

Coolant:
Frozen gel packs,dry ice if already frozen
Fee:
62.00
Days Tested:
First Tuesday of the month
Turnaround Time:
One month
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.

Specimen Requirements:

1) feces 2) fecal swab 3) fresh small intestine 4) viral isolate

Shipping Container:

1,3) whirl-pac or leakproof container 2) swab

Coolant:
frozen gel packs
Fee:
269.00
Days Tested:
as needed
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) feces 2) fecal swab 3) fresh small intestine 4) viral isolate

Shipping Container:

1,3) whirl-pac or leakproof container 2) swab

Coolant:
frozen gel packs
Fee:
142.00
Days Tested:
as needed
Turnaround Time:
2-4 weeks
Comments:

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. If multiple agent testing is requested, please submit 2 tablespoons/30ml of fecal material. This will allow for an adequate sample volume to properly conduct each test.When sequencing is requested and a particular sample or number of samples to sequence isn't specified, the lab will default to sequencing the one sample with the lowest ct value (the lowest ct value represents the sample with the most virus or bacteria).

Specimen Requirements:

1) Tendon (at least 1 cm);  2) heart (at least 1 cm3);  3) semen (at least 0.5 ml)

Shipping Container:

1,2) whirl-pac or plastic bag;  2) plastic tube

Coolant:
Frozen gel packs
Fee:
115.00
Days Tested:
F
Turnaround Time:
5 business days for PCR; 3-6 weeks for viral isolation
Comments:

The PCR portion of this test is run at the Minnesota Poultry Testing Laboratory. The virus isolation is conducted in the St. Paul facility. 

This panel includes a turkey-specific qPCR (will not detect chicken Reovirus) and 2 types of viral isolation (using egg inoculation and cell lines). We expect to isolate virus from any sample with an initial positive PCR result. An initial negative PCR result, however, will require confirmation via isolation results to be confident in the results of the assay.

Turkey Reovirus (TRV) isolation may be requested independently, but any request for TRV PCR will also include viral isolation (at the panel price of $115).

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. 

Specimen Requirements:

1) tissue 2) culture media

Shipping Container:

sterile container

Coolant:
frozen gel packs
Fee:
15.00
Days Tested:
F
Turnaround Time:
Results reported same day of test (F)
Comments:

RT-PCR or qRT-PCR. VHS Surveillance Study The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are to be requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test.