Virology

Services & Fees

Effective January 1 2018

Note: A $10 accession fee will be applied to all submissions. All fees are in US $.

Note on days tested and turnaround times: Samples must be received at least one business day before the scheduled testing day. New client accounts can take up to 2-3 business days to establish before testing can begin. Samples may not always be tested on the first scheduled day after they arrive; however, it is our goal to complete testing within the posted turnaround time. If rush testing is desired, please contact us.

Submission form

Click the name of the test or procedure for detailed information.

Aquaculture Virus Inspection and Virus Isolation (Fish)

Specimen Requirements:

live or freshly dead fish

Shipping Container:

leakproof container or plastic bags

Coolant:
frozen gel packs
Fee:
216.00 per lot per cell line per temperature (60 fish)
Days Tested:
M-W
Turnaround Time:
14-35 days
Comments:

Additional pools (up to 5 fish) are $18.00. 

Avian Reovirus Panel (PCR and Virus Isolation)

Specimen Requirements:

1) Tendon (at least 1 cm);  2) feces (at least 1 gram);  3) yolk (at least 0.5 ml); 4) cell culture (at least 0.5 ml)

Shipping Container:

1) whirl-pac or plastic bag;  2-4) plastic tube

Coolant:
Frozen gel packs
Fee:
115.00
Days Tested:
F for PCR; weekly for viral isolation
Turnaround Time:
5 business days for PCR; 3-6 weeks for viral isolation
Comments:

The PCR portion of this test is run at the Minnesota Poultry Testing Laboratory. The virus isolation is conducted in the St. Paul facility. 

This panel includes a real-time PCR and 2 types of viral isolation (using egg inoculation and cell lines).

The specimen type(s) listed for this test will provide an adequate sample volume to conduct this test. If multiple tests are requested on a specimen, there may not be adequate sample volume to perform each test. Please submit an adequate sample volume to meet the requirements of each test. 

BVD Antigen Capture ELISA (ACE)

Specimen Requirements:

1 cm x 1 cm ear tissue (1/2 inch notching pliers)

Shipping Container:

6 or 10 ml red top tube (no additive or gel)

Coolant:
frozen gel packs
Fee:
5.25
Days Tested:
W
Turnaround Time:
2-8 bus. days
Comments:

For individual testing of ear notch samples only. See Molecular Diagnostics, Viral PCR for BVD ear notch pooled testing.

Rabies Extraction/Preparation

Specimen Requirements:

head or whole brain

Shipping Container:

sealed, leakproof container

Coolant:
frozen gel packs
Fee:
20.00 (per animal) plus $10.00 accession fee
Days Tested:
M-F
Turnaround Time:
1-2 business days
Comments:

See the Rabies home page for more information on submitting for this test. After the brain samples are collected at the VDL, staff at the Minnesota Department of Health perform the fluorescent antibody test.

Turkey Reovirus - VI Only Egg Inoculation

Specimen Requirements:

Tendon, heart

Shipping Container:

whirl pac or plastic bag or plastic tube

Coolant:
Frozen gel packs
Fee:
53.00
Days Tested:
weekly
Turnaround Time:
3 -6 weeks
Comments:

Turkey Reovirus VI only Cell Line

Specimen Requirements:

Tendon, heart

Shipping Container:

whirl pac or plastic bag or plastic tube

Coolant:
Frozen gel packs
Fee:
35.00
Days Tested:
Weekly
Turnaround Time:
3 -6 weeks
Comments:

All samples are inoculated both in cell culture and embryonated chicken eggs.

Virus Isolate Send out (Preparation & Shipping)

Specimen Requirements:

isolate

Shipping Container:
Coolant:
Fee:
104.00
Days Tested:
as requested
Turnaround Time:
7-14 business days*
Comments:

Once ready, isolates are shipped M-Th from the laboratory. No shipping on Fridays.

*The turnaround time may vary depending upon growth of virus in cells.

Virus Isolation (Avian - In Embryonated Eggs)

Specimen Requirements:

tissue, swabs, etc

Shipping Container:

plastic tube or plastic bag

Coolant:
frozen gel packs
Fee:
35.00
Days Tested:
T
Turnaround Time:
21-28 days or more if blind passages are needed (see comments)
Comments:

Turnaround time will change if any case(s) have to be reinoculated for any reason. See Appendix A for a list of cell types used to isolate viruses.

Virus Isolation (Fish)

Specimen Requirements:

tissue

Shipping Container:

plastic tube or plastic bag

Coolant:
frozen gel packs
Fee:
28.00 per cell line per temperature
Days Tested:
T-W
Turnaround Time:
14-21 days
Comments:

See Appendix A for a list of cell types used to isolate viruses.

Virus Isolation (Mammalian)

Specimen Requirements:

tissue, swabs, feces or buffy coat

Shipping Container:

plastic tube or plastic bag

Coolant:
frozen gel packs
Fee:
35.00 each cell line
Days Tested:
F
Turnaround Time:
14 -21 days or more if blind passages are needed
Comments:

See Appendix A for a list of cell types used to isolate viruses.

Virus Isolation (Senecavirus A - SVA (Seneca Valley Virus)

Specimen Requirements:

tissues, swabs, vesicular fluids, etc.

Shipping Container:

plastic tube or plastic bag

Coolant:
frozen gel packs
Fee:
35.00
Days Tested:
Once weekly or as needed
Turnaround Time:
14-21 days or more if blind passages are needed
Comments:

See Appendix A for a list of cell types used to isolate viruses.

Virus Isolation (Swine Influenza Virus)

Specimen Requirements:

tissue, swabs, etc.

Shipping Container:

plastic tube or plastic bag

Coolant:
frozen gel packs
Fee:
35.00 each cell line
Days Tested:
T-Th
Turnaround Time:
14-21 days or more if blind passages are needed
Comments:

See Appendix A for a list of cell types used to isolate viruses.

Virus Titration - Swine Influenza Virus (SIV), Porcine Reproductive and Respiratory Syndrome (PRRS) Virus

Specimen Requirements:

virus isolate or sample

Shipping Container:

styrofoam

Coolant:
frozen gel packs
Fee:
91.00
Days Tested:
on request as needed
Turnaround Time:
14-28 days
Comments:

The turnaround time may vary depending upon availability of cells, growth of virus in cells, more number of samples, etc. The client will be notified if turnaround time exceeds the stipulated time.