Electron Microscopy Submission

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Pathogen Identification

Clinical cases

  • Sample type (table) and quantity:
  • Secretion, exudates, lavage, fluid, and feces: >0.3 ml
  • Papule and tissue: >0.3mm (biopsy)
  • Sample collection:
  • Sample must be collected in any available sterile vial and submitted fresh or refrigerated at 4 degrees C.
  • Sample must be correctly identified with case number, animal species, type of sample, date of collection, and submitter contact information.

Cultures isolate (Please contact Dr. Aníbal G. Armién):

  • Known agents (samples with control cell line for negative stain)
  • Unknown agents (samples with control cell line for negative stain and isolate embedding)

Tissue Ultrastructure

  • Tissues fixed as soon as possible in one of the following EM fixatives (optimal method):
  • 2.5% Glutaraldehyde, 0.1M Sodium Cacodylate buffer
  • 2.0% Paraformaldehyde/ 0.5% Glutaraldehyde, 0.1M Sodium Cacodylate buffer
  • Karnovsky’s Solution

Biopsy or any tissue collected from a fresh animal carcass should be placed in EM fixative, ideally within six hours after death.

  • Tissue fixed in 10% Neutral Buffered Formalin (NBF)
    Biopsy or any tissue collected from a fresh animal carcass should be placed in 10% NBF, ideally within a period of three hours after death. No tissue size is required.
  • Paraffin-embedded tissue
    Tissues fixed in NBF from biopsy or a fresh animal carcass, ideally collected within a period of one hour after death.

Sample Submission  

Please submit samples using our submission form, with full information:

  • Disease history
  • Pathology report and diagnosis
  • H & E slide (if available)

Collaborations

Collaboration and co-authorship: The Diagnostic Ultrastructural Pathology Service offers a full diagnostic service; selected staff members of the Ultrastructural Pathology Section are requested to be considered as co-authors when data generated in our service are being published. 

Materials and methods: Please contact us for manuscript preparation (methods and materials, results sections – e.g., description, images).

Contact

Dr. Aníbal Armién, Diplomate ACVP
Pathologist
Room 236, Veterinary Diagnostic Laboratory
1333 Gortner Ave.
St. Paul MN 55108

E-mail: armie001@umn.edu
Phone: 612-625-8787
Toll Free: 800-605-8787