Bacterial Whole Genome Sequencing
Service type
Molecular Development
Fee
$249.60
Days tested
As needed
Turnaround time
2-4 weeks
Specimen Requirements
1) At least 400 uL (0.4 mL) 2) agar plate with fresh growth
Shipping container
1) plastic tube with secure cap; 2) agar plate (not recommended for shipping)
Coolant
Dry-ice preferred, ice packs acceptable (thawed or mishandled samples will affect the quality of NGS results)
Location Term
St. Paul (VDL)
Submission Form(s)
Best Practice
Description
Note: A bacterial culture will be performed first and billed to the client for any external samples submitted for sequencing
Available for bacterial isolates only. We use Illumina technology and aim for 30-50x coverage depth of the bacterial genome. Analysis includes de novo assembly, multi-locus sequence typing (MLST), antibiotic resistance gene screening, virulence gene screening, pangenome comparison, SNPs and pangenome based dendrograms. Specific analysis such as serotyping can be performed based on needs.